With the monoclonal antibody against kanamycin being prepared successfully, a bead-based indirect competitive fluorescent immunoassay was developed to detect kanamycin in milk.The fact that there was no significant cross-reaction with other aminoglycoside antibiotics implied that the monoclonal antibody was Vase highly specific for kanamycin.The limit of detection (LOD) and the 50% inhibition concentration (IC50) in raw milk were 3.
2 ng/mL and Scrappers 52.5 ng/mL, respectively.Using the method developed in this study, the kanamycin concentrations were monitored in raw milk after the intramuscular administration of kanamycin in sick cows.
Compared to the conventional enzyme-linked immunosorbent assay (ELISA), the method using the suspension array system was more sensitive.The results obtained in the present study showed a good correlation with that of the ELISA.